Figure 6. Spleen-derived macrophages recapitulate the differential pattern of cytokine and chemokine production observed in BMDM from C57 and C3H mice, as well as the effects of exogenous and endogenous IL-10.

Spleen-derived macrophages (SDM) were stimulated and incubated as described in Figs. 1, 4 and 5. IL-6 and CCL5 were detected in cell-free supernatants from SDM cultures using cytokine-specific ELISAs. Statistical analysis was performed as indicated in Fig. 2. Significance was established at P<0.001 = ***. Each bar represents the mean ± standard deviation for duplicate cultures. These experiments were repeated two times, with similar results.