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TK-173 and HK-2 cells were treated with Ang II, PDGF-AB and TGF-ß1 for 24 hours. After RNA isolation all 4 versican isoforms were analyzed by real-time PCR (n = 3 assays per ligand). Results shown are mean ratios to the housekeeper gene PPIA ± standard deviation. *p<0.05 as compared to controls.
