Figure 5. Klf5 contributes to corneal epithelial homeostasis by regulating the expression of desmogleins and desmoplakin.

(A) Immunofluorescence shows abundant expression of desmogleins (red, panel ii) and desmoplakin (red, panel v) in the WT but not in the Klf5CN corneal epithelium (panels iii and vi, respectively). Sections processed in a similar manner but without the primary antibody served as negative controls (panels i and iv). Scale bars = 25 µm. (B) Relative activities of 2 kb Dsg1a, Dsg1b and Dsp promoter fragments measured by transient co-transfection assays with or without Klf4, Klf5, or both in NCTC cells. Error bars indicate Standard Error of Mean (SEM). Data are representative of three independent experiments.