Cell model with two different parameterizations shown in “z-stack” form. The parameters σx, σy, and H vary, but are constrained so that the total volume above the glass slide inside the cell is held constant. (a) θ = 0, σx = 5, σy = 5, H = 8 and (b) θ = 0, σx = 4, σy = 6.25, H = 8.