Fig. 4.
RvE1 enhances resolution of E. coli–evoked pneumonia. Six hours after intratracheal instillation of 107 live E. coli, mice were treated with vehicle or RvE1 (25 μg/kg, i.p.) and/or the pan-caspase inhibitor zVAD-fmk (10 μg/kg, i.p. three times at 4-h intervals). Mice were killed 24 h later and BAL fluid total leukocyte (A), neutrophil (B), and monocyte/macrophage counts (C), the percentage of annexin-V–positive (apoptotic) neutrophils (D), the percentage of neutrophils with decreased mitochondrial transmembrane potential (ΔΨm) (E), BAL cell cytoplasmic histone-associated DNA fragments (F), and the number of BAL fluid macrophages containing apoptotic bodies (arrows, Left) (G) were determined. Data are means ± SEM (n = 6–8 mice per group). *P < 0.05, **P < 0.01; ***P < 0.001.