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Bisphenol-induced coactivator recruitment and structural dynamics. (A) Titration of fluorescein-labeled SRC-1 NR2 peptide by ERα LBD in the absence of ligand or in the presence of E2 (agonist), OHT (antagonist), BPA, BPAF, or BPC. (B) Anisotropy measurements of fluorescein-labeled ERα LBD in the presence of saturating concentrations of bisphenols, E2, or OHT. (C) Similar experiments performed in the presence of increasing concentrations of the coactivator-derived peptide SRC-1 NR2.
