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. 2012 Aug 20;109(37):15042–15047. doi: 10.1073/pnas.1206273109

Fig. 1.

Fig. 1.

Separation and molecular mass of ovulation-inducing factor (OIF) in llama seminal plasma. (A) Separation of fraction C was done using sephacryl gel filtration fast protein liquid chromatography and isocratic elution with PBS. Fraction C was isolated previously by hydroxylapatite column chromatography. (B) Protein band at about 14 kDa on denaturing 12% SDS/PAGE (circled) was the major constituent of fraction C2. (C) Mass spectra (mass-to-charge ratio; m/z) of OIF (fraction C2 isolated from llama seminal plasma) by MALDI-TOF analysis showing a single peak at 13,221 Da, corresponding to the monomeric subunit of the homodimer of β-NGF. (D) Narrow peak appeared at 16.755 mL of elution volume after loading a nondenatured sample of fraction C2 into a calibrated superdex gel filtration column, corresponding to the molecular mass of β-NGF (i.e., homodimer of 26 kDa).