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. 2012 Sep 14;7(9):e45045. doi: 10.1371/journal.pone.0045045

Figure 4. SAHA treatment promoted the expression of EMT-related factors and acetylation of histone 3 at different time points.

Figure 4

(A) Total RNA was isolated from LNCaP cells treated with 2.5 µM and 5 µM of SAHA for 8, 16 and 24 h. The results from real time RT-PCR showed that the relative mRNA expression of transcription factors such as ZEB1 and Slug was up-regulated as early as 8 h of treatment with SAHA, while vimentin and N-Cadherin was increased after 16 h of treatment compared to DMSO control (the value of control was designed as 1, *, p<0.05; **, p<0.01). (B) LNCaP cells were treated with 5 µM SAHA for 8 h to 72 h. Western blot analysis showing that SAHA treatment promoted acetylation of histone3 (Ac-H3) at 8 h to 72 h treatment. Fibronectin (Fibron) significantly increased after 24 h of treatment and vimentin was dramatically elevated after 16 h of treatment with SAHA. (C) PC3 cells were treated with 5 µM SAHA for 4 h to 32 h. and increased expression of ZEB1 was observed at 4 h to 32 h treatment, which was associated with Hyper-acetylation of histone 3. Enhanced expression of vimentin was observed at 32 h of treatment with SAHA.