Skip to main content
. 2012 Sep 14;7(9):e45440. doi: 10.1371/journal.pone.0045440

Figure 7. Differential targeting of GTPase Irgb6 to parasitophorous vacuoles of type I and type II Toxoplasma strains in SkMCs.

Figure 7

Differentiated C2C12 SkMCs were infected with T. gondii RHβGal or NTE parasites and were activated with 100 U/ml IFN-γ at the time of infection. After fixation at different time points p.i., Irgb6 (green fluorescence) and T. gondii (red fluorescence) were immunolabeled and analysed by confocal laser scanning microscopy. (A) Representative images of PVs of NTE and RHβGal parasites from one out of two independent experiments are depicted. (B) Percentages of Irgb6-positive parasitophorous vacuolar membranes (PVM) were determined microscopically by examining at least 100 vacuoles per sample; data represents means ± S.E.M. (n = 2). (C) Irgb6-positive RHβGal vacuoles could be rarely observed; a RHβGal vacuole showing early signs of disruption is indicated.