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. 2012 Jul 31;3(7):700–708. doi: 10.18632/oncotarget.587

Figure 5. The effect of dominant-negative PAK1 on growth of SK-MEL-103 cells in confluent cultures.

Figure 5

A. SK-MEL-103 cells transduced with a construct expressing dominant-negative PAK1-K299R (“dnPAK”) or the respective vector control (“vector”) were cultured for two days after reaching confluence. Three wells of each variant were fixed and stained with Hoechst 33342, and readings were taken from 25 points on each of the wells. The average value (in arbitrary units) of all the readings for each cell line is shown with standard deviation. B-C. The indicated cell lines were cultured for six more days after reaching confluence, fixed and stained with Hoechst 33342 and photographed under a fluorescent microscope. Note the change from uniform multilayer growth to individual foci.