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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Aug 12;19(9):925–929. doi: 10.1038/nsmb.2358

Figure 3. Three-dimensional structures of two types of kinetochore particles bound to a microtubule.

Figure 3

a and b, projection images of the two types of kinetochore assemblies on taxol-stabilized microtubules selected for tomographic reconstruction. Representative slices through the each particle are presented with the corresponding segmentation analysis in color underneath. Finally, 3D reconstruction of this complex by electron tomography is presented on the right. All slices are arranged from left to right traversing through the z-axis from bottom to top with the bottom defined at the grid surface (carbon layer). Both structures contain a ring-like structure (blue arrowhead) and multiple rods (white arrowhead). Scale bars for all panels represent 100 nm. Movies are presented in Supplementary Material.