Fig. 2.

(A) Representative flow cytometry profile of lymphocytes following 3-day co-culture with virus infected DCs. CD4-gated lymphocytes expressing CD25⁺ and FoxP3⁺ are shown. (I) Uninfected control DCs; (II) PRRSV-infected DCs; (III) PCV2-infected DCs; and (IV) PRRSV/PCV2 co-infected DCs. (B) Mean CD4⁺CD25⁺FoxP3⁺ T cells ± standard error of the mean as a percentage of lymphocytes co-cultured with DCs infected with PRRSV, PCV2 or both viruses. Data represent three replicates per group from five independent experiments (n = 5 pigs). Data not connected by the same letter are significantly different, p < 0.05.