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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2012 Jul 15;18(10):1488–1499. doi: 10.1016/j.bbmt.2012.07.009

Figure 2. Generation of allospecific TEM.

Figure 2

Figure 2

To generate allospecfic TEM, TEa spleen cells containing 1×107 TEa T cells were first transferred into Rag-1−/− mice. These mice were subsequently immunized with irradiated CB6F1 splenocytes 18–24 hour later. (A) A schema showing how effector memory TEa cells were generated. The details of how allospecific TEM were obtained are described in the Material and Methods section. (B) Phenotypes of TEa cells TEM donor mice. Flow cytometric analysis was performed in TEM donor mice at least 8 weeks after priming. (C) TEM donor mice mediated “second-set” skin graft rejection. At least 8 weeks after priming, TEM donor mice were transplanted with CB6F1 skin grafts. Graft survival was observed daily. P<0.01, TEM donors vs. other groups. P=0.057, TEM-like donors vs. naïve TEa mice.