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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2012 Jul 15;18(10):1488–1499. doi: 10.1016/j.bbmt.2012.07.009

Figure 6. Allospecific TEM undergo apoptosis and die faster than naïve T cells do in vitro.

Figure 6

Purified TEa cells from different groups were labeled with CFSE. Labeled TEa cells (1.25×105/well) were cultured with irradiated CB6F1 spleen cells (5×105/well) for 3 days. At the end of the culture, cells were stained with Anexin V and 7-AAD. The dot plots were gated on 7-AAD− (top panel) or total (bottom panel) CD229.1−CD4+Vα2+ (TEa) cells. The values represent percent apoptotic (AnexinV+7-AAD−) or dead cells (7-AAD−) among TEa cells. A representative of three similar experiments with similar results is shown.