Figure 7. Allospecific TEM are deleted in ‘GVHD’ but not skin graft recipients.
Spleen cells from skin graft recipients (Figure 2C) and “GVHD” recipients (Figure 4) were harvested 84 days after transplantation. Proliferative responses against alloantigen and the numbers of TEa cells in peripheral blood were determined. (A) Proliferation against alloantigens. Various numbers of spleen cells were cultured with irradiated CB6F1 spleen cells (5×105/well) for 3 days. Proliferation was determined by 3H-thymidine incorporation. Results are represented as mean±SD of triplicate wells. All experiments were repeated at least twice and yielded similar results. *P<0.05. (B) Peripheral TEa cell counts. Absolute counts of TEa cells were determined by flow cytometer. Each group contained at least 5 animals. *P<0.01.