Fig. 5.
N-type calcium channel (N-CC) immunostaining (a: × 100, b – f: × 200). N-CC was strongly expressed in distal nephron and podocyte (a). The area positive for N-CC immunoreactivity in glomeruli was similar between WKY (f) and vehicle group (b). Cilnidipine significantly decreased N-CC immunostaining in the glomeruli of both the denervated (e) and innervated (d) groups compared to the vehicle group (b). Denervation did not attenuate N-CC immunostaining in the glomeruli of the cilnidipine-treated (e) and untreated (c) groups. All rats were uninephrectomized and fed the 4% NaCl– containing diet. #P < 0.05 vs. the vehicle group. WKY: Wistar Kyoto rat, DNX: denervation. Scale bars: 100 μm.