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. Author manuscript; available in PMC: 2013 Sep 11.
Published in final edited form as: Dev Cell. 2012 Aug 16;23(3):533–546. doi: 10.1016/j.devcel.2012.06.008

Figure 3. Identification of IQGAP and cortexillins as α-catenin-interacting proteins.

Figure 3

A) Whole fruiting body lysates were immunoprecipitated with control or anti-α-catenin antibodies and the resulting samples analyzed by SDS-PAGE and silver staining.

B) Partial list of proteins identified by LC/MS-MS analysis of an α-catenin immuoprecipitate from whole fruiting bodies.

C–E) Confocal sections of the entire tip (upper panels) or tip epithelium (lower panels) of wild-type culminants stained as indicated. In the lower panels, the top of the image faces the stalk. Arrows indicate the apical membrane, where actin and myosin are enriched but IQGAP1, Cortexillin I and II and α-catenin are absent. Scale bars represent 10 μm in upper panels and 2 μm in lower panels.

See also Figure S3.