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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Dev Comp Immunol. 2012 Jul 31;38(2):275–284. doi: 10.1016/j.dci.2012.06.012

Fig. 7. Recombinant MsGlv binds to the O-specific antigen and outer core carbohydrate moieties of LPS.

Fig. 7

Wells of 96-well fat-bottom microtiter plates were coated with smooth LPS from several bacteria, Ra-, Rc-, Rd- and Re-LPS, monophosphoryl and diphosphoryl lipid A. Recombinant MsGlv and GFP purified from Drosophila S2 cells were diluted to 120 nM and added to the ligand-coated plates, and the binding assay was performed as described in the Materials and Methods. The figure showed specific binding of recombinant MsGlv to LPS and lipid A after subtracting the total binding of the control GFP from the total binding of MsGlv. Each bar represent the mean of three individual measurements ± SEM. Comparing binding of recombinant MsGlv to different ligands, identical letters indicate not significant difference (p>0.05), while different letters indicate significant difference (p<0.05) determined by one way ANOVA followed by a Tukey's multiple comparison test.