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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Dev Comp Immunol. 2012 Jul 31;38(2):275–284. doi: 10.1016/j.dci.2012.06.012

Fig. 8. Antimicrobial activities of recombinant MsGlv.

Fig. 8

Mid-log phase bacteria (Gram-negative E. coli and S. marcescens, Gram-positive B. cereus, B. subtilis, B. thuringiensis and S. aureus) and fungi (S. cerevisiae (BY4741) and C. neoformans (alpha)) were diluted to OD600 = 10-5 and incubated with recombinant MsGlv purified from S2 cells (final concentration of ~21 μg/ml or 1.5 μM) or water (Control) in 96-well plates with 220 rpm shaking at 37°C (for bacteria) or 30°C (for fungi). OD600 was recorded every hour up to 20 h (for bacteria) or 30 h (for fungi) after incubation. The points represent the mean of four individual measurements ± SEM. The asterisks in the boxes indicate inferior but significant activity (p<0.05) between MsGlv and the control at the indicated time points determined by an unpaired t-test.