Analysis of subcutaneous lymph node and splenic DCs from VDR WT and VDR
KO mice between 6 and 12 weeks old. (A) Lymph nodes from
KO mice were uniformly larger than those of WT (representative example
from one pair of animals). (B) Two-color flow cytometric
analysis of lymph node (○) and spleen
(●) cells from six separately analyzed WT and KO
pairs by using stains for CD11c, class II MHC (I-Ab), CD40,
and CD80/CD86. Lines connect values for the WT and KO member of each
individual pair. P values by paired and nonpaired
analysis for differences between WT and KO animals were: lymph
node—%CD11c+ve (P = 0.6 and 0.77),
I-Ab MFI (P = 0.01 and 0.016), CD40 MFI
(P = 0.003 and 0.012), CD80/CD86 MFI
(P = 0.03 and 0.33); spleen—%CD11c+ve
(P = 0.05 and 0.18), I-Ab MFI
(P = 0.4 and 0.7), CD40 MFI (P
= 0.3 and 0.8), CD80/CD86 MFI (P = 0.05 and
0.06). (C) An example is shown of flow cytometric
analysis of lymph node cells from a VDR WT and KO pair. Class II MHC
(I-Ab) expression by CD11c+ve cells is shown.
Within the CD11c+ve cells from both animals populations
with low, intermediate, and high expression of I-Ab are
present. For the KO animal the I-Ab high cells (encircled)
represent the predominant population with relative reductions in the
I-Ab low and intermediate cells compared with the WT
animal.