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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Cell Microbiol. 2012 Jun 26;14(10):1497–1512. doi: 10.1111/j.1462-5822.2012.01823.x

Figure 1.

Figure 1

Non-transfected HeLa cells (A, C, and D) or HeLa cells transfected with the plasmid encoding ABCA1-EGFP (B) were infected with Chlamydia trachomatis serovar D. The cells were fixed at 24 hours (A, C, and D) or 48 hours (B) PI with 4% paraformaldehyde in PBS (A - C) or with methanol (D). The cells in A - C were then permeabilized in PBS containing 0.1% Triton X-100 and incubated with a mouse monoclonal antibody directed against IncA (A - C) and a rabbit polyclonal antibody directed against ABCA1 (A and C). Alternatively, the cells in D were directly incubated with a monoclonal antibody directed against CT223 and a rabbit polyclonal antibody directed against ABCA1. Following washing, the cells were incubated with the appropriate secondary antibodies prior to confocal analysis. The arrow in C points to a membrane aggregate between adjacent inclusions that is positive for IncA and ABCA1. The white bars in A and D are 5μm, the bar in B is 10μm, and the bar in C is 2.5μm.