Figure 5.

HeLa cells infected with Chlamydia trachomatis serovar D were incubated in the presence of 1.5μM β-BODIPY FL C₅-HPC in fatty acid-free BSA for 1 hour prior to fixation in 4% paraformaldehyde in PBS at 48 (A) or 24 (B) hours PI. The labeled cells were then permeabilized by a brief incubation in methanol and incubated with a monoclonal antibody directed against IncA and a rabbit polyclonal antibody directed against apoA-1. Following incubation with appropriate secondary antibodies, the cells were analyzed by confocal microscopy. The arrow in B indicates the perinuclear pool of β-BODIPY FL C₅-HPC in an infected cell. The white bars are 5μm.