. Author manuscript; available in PMC: 2013 Nov 1.

Published in final edited form as: J Pharm Biomed Anal. 2012 May 23;70:320–329. doi: 10.1016/j.jpba.2012.05.008

Table 3.

Calibration curves of bupropion and its three metabolites in umbilical cord plasma and placental tissue (n=3).

Bio-matrix	Analyte^a	Regression equation^b, ^c	r²	F-test^d		Linear range^c	LOD^c
Bio-matrix	Analyte^a	Regression equation^b, ^c	r²	F	p-Value	Linear range^c	LOD^c
Umbilical cord plasma	BUP	y=0.007+0.074x	0.995	0.79	0.614	0.250-250	0.050
	OH-BUP	y=0.010+0.089x	0.996	1.02	0.434	0.715-715	0.072
	EB	y=0.004+0.181x	0.996	1.01	0.440	0.245-245	0.049
	TB	y=0.022+0.183x	0.995	1.00	0.447	0.365-365	0.073
Placental tissue	BUP	y=0.011+0.110x	0.992	1.31	0.301	0.312-250	0.125
	OH-BUP	y=0.023+0.174x	0.991	1.33	0.294	0.895-716	0.179
	EB	y=0.005+0.199x	0.991	1.09	0.411	0.305-244	0.122
	TB	y=0.015+0.132x	0.989	1.41	0.262	0.918-734	0.184

BUP=buproipion, OH-BUP=hydroxybupropion, EB=erythrohydrobupropion, TB=threohydrobupropion.

In the regression equation y=b+ax, x refers to the concentration of the analyte in bio-matrix; y refers to the peak area ratio of the analyte vs. IS.

The unit of analyte in umbilical cord plasma is ng/mL; the unit of analyte in placental tissue is ng/g.

F-test for lack-of-fit.