. Author manuscript; available in PMC: 2013 Nov 1.

Published in final edited form as: J Pharm Biomed Anal. 2012 May 23;70:320–329. doi: 10.1016/j.jpba.2012.05.008

Table 6.

Stability of bupropion and its three metabolites in umbilical cord plasma and placental tissue (n=3)

Bio-matrix	Analyte^a	Added concentration^b	Freeze and thaw stability (3cycles, −80°C)			Short-term stability (4h, 22–25°C)			Long-term stability (30days, −80°C)
Bio-matrix	Analyte^a	Added concentration^b	Mean concentration obtained^b	Mean accuracy^c (%)	Precision (RSD, %)	Mean concentration obtained^b	Mean accuracy^c (%)	Precision (RSD, %)	Mean concentration obtained^b	Mean accuracy^c (%)	Precision (RSD, %)
Umbilical cord plasma	BUP	0.625	0.606	96.9	4.5	0.601	96.2	6.2	0.629	100.6	11.8
		188	172	91.9	6.9	172	92.0	3.1	203	108.3	7.1
	OH-BUP	1.79	1.74	97.5	10.7	1.75	97.7	3.0	2.02	112.8	11.6
		536	494	92.1	5.0	492	91.8	2.3	553	103.1	7.0
	EB	0.612	0.549	89.6	7.7	0.560	91.5	6.2	0.653	106.7	12.5
		184	166	90.5	3.1	166	90.7	2.7	193	104.8	7.8
	TB	0.912	0.814	89.2	6.4	0.882	96.7	5.3	0.991	108.6	12.0
		274	249	91.1	3.3	254	93.0	2.9	259	104.4	7.7
Placental tissue	BUP	0.625	0.571	91.4	1.7	0.622	99.5	5.2	0.659	105.4	5.5
		188	193	103.0	4.8	192	102.7	5.8	186	99.1	2.3
	OH-BUP	1.79	1.65	91.7	3.8	1.78	99.5	2.3	1.73	97.0	5.2
		536	539	100.3	4.1	536	99.8	4.9	510	95.2	1.3
	EB	0.610	0.555	91.0	4.8	0.586	96.1	7.1	0.617	100.7	5.7
		183	191	104.4	6.1	186	101.8	5.2	179	97.2	5.7
	TB	1.84	1.74	95.0	4.0	1.81	99.1	2.9	1.73	94.5	5.2
		550	522	100.3	4.4	547	99.4	3.6	508	92.2	8.3

BUP=buproipion, OH-BUP=hydroxybupropion, EB=erythrohydrobupropion, TB=threohydrobupropion.

The unit of analyte in umbilical cord plasma is ng/mL; the unit of analyte in placental tissue is ng/g.

accuracy=(obtained concentration/added concentration)×100