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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: Dev Biol. 2012 Jul 25;370(2):165–172. doi: 10.1016/j.ydbio.2012.07.020

Fig. 2. Estimating the required Hill coefficient to pattern Hb profiles in embryos of different stages or backgrounds (Lopes et al., 2008).

Fig. 2

The Hill coefficient is given by the inclination of the straight line (see equation (2) in Material and Methods). (A): Plot from a WT embryo at early cycle 14A exhibiting an nH of 6.3 that is similar to the mean nH (6.3, s.d. 1.4) measured from 15 embryos at this stage (inset). (B): The same plot as in (A) but from a WT embryo at late cycle 14A with an nH of approximately 11.6 that is similar to the mean nH (10.8, s.d. 4.0) measured from 15 embryos at this stage (inset). (C): Same plot as in (A) but from an hb14F embryo at cycle 14A with an nH of approximately 3.0. Inset: 15 embryos at this stage with the mean nH (3.0, s.d. 1.0). (D): The same plot as above from an embryo expressing the pThb5 lacZ artificial construct at cycle 14A and exhibiting an nH of 7.0. Inset: 9 embryos with mean nH (7.0, s.d. 1.5). We assumed early and late cycle 14A embryos corresponded to stages T1-T4 and T5-T6, respectively, according to previous classification (Surkova et al., 2008).