Figure 1.
Human adipose tissue-derived mesenchymal stem cell (ATSC) viability and proliferation. Human ATSCs were cultured in the presence of either GY785 DR (A and C) and GY785 DRS (B and D) at the indicated concentrations or Act (5 μg/ml) for 72 hours. Viability (A and B) was evaluated by MTS activity measurement and expressed as the relative MTS activity compared to the untreated control. Proliferation (C and D) was estimated by viable cell counting after trypan blue exclusion dye. *p < .05 compared to the control condition (0 μg/ml of polysaccharide). Abbreviations: Act, actinomycin D; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt.