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. 2011 Nov 30;30(3):471–480. doi: 10.1002/stem.1686

Figure 2.

Figure 2

Biochemical characterization of human adipose tissue-derived mesenchymal stem cell (hATSC) pellets. hATSCs were cultured in pellets in the presence of CT or CH medium supplemented or not with 50 μg/ml or the indicated concentrations of GY785 DR or GY785 DRS for 28 days. (A): The pellet volumes were calculated by equating these entities to ellipsoids. #p < .05 compared to the control condition. GAG production was evaluated at a gross level after Alcian Blue staining of the whole pellets. Bar: 500 μm. (B): Total DNA contents were evaluated using a DNA quantification kit. Results are expressed as the relative content compared to the control condition. #p < .05 compared to the control condition. *p < .05 compared to the chondrogenic condition. (C): Total collagens contents were quantified using a Sircol Kit. Results are expressed as the relative content compared to the control condition. #p < .05 compared to the control condition. *p < .05 compared to the chondrogenic condition. (D): GAG contents were quantified using a Blyscan Kit. Results are expressed as the relative content compared to the control condition. #p < .05 compared to the control condition. *p < .05 compared to the chondrogenic medium supplemented with GY785 DR at the same concentration. Abbreviations: CH, chondrogenic; CT, control; GAG, glycosaminoglycan.