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. 2011 Nov 30;30(3):471–480. doi: 10.1002/stem.1686

Figure 3.

Figure 3

Histological characterization of human adipose tissue-derived mesenchymal stem cell (hATSC) pellets. (A): Human ATSCs were cultured in pellets for 28 days in the presence of CT or CH medium supplemented or not with GY785 DR or GY785 DRS (50 μg/ml). Histological sections of hATSC pellets were stained with HES (a, c, e, g, i, and k) and Alcian Blue (b, d, f, h, j, and l) as indicated in Materials and Methods. Bar: 100 μm. (B): Human ATSCs were cultured in pellets for 0, 14, and 28 days in the presence of chondrogenic medium supplemented with GY785 DRS (50 μg/ml). Histological sections of hATSC pellets were stained with Alcian Blue (a, e, and i) and immunostained for type I (b, f, and j), II (c, g and k), and X (d, h, and l) collagens as indicated in Materials and Methods. Bar: 100 μm. Abbreviations: CH, chondrogenic; CT, control; HES, hematoxylin-eosin-safran.