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. 2011 Nov 30;30(3):471–480. doi: 10.1002/stem.1686

Figure 4.

Figure 4

Analysis of the expression levels of chondrogenic markers. (A): Human adipose tissue-derived mesenchymal stem cell (ATSC) pellets were cultured in the presence of CT or CH medium supplemented or not with 50 μg/ml of GY785 DR or GY785 DRS for 28 days. Expression of the chondrogenic markers COL2A1, ACAN, SOX9, and COMP was investigated by real-time reverse transcription-polymerase chain reaction (RT-PCR). Results are expressed as relative expression level compared to the chondrogenic medium in the absence of polysaccharide. *p < .05 compared to chondrogenic medium. #p < .05 compared to chondrogenic medium supplemented with GY785 DR. (B) Human ATSC pellets were cultured in the presence of chondrogenic medium (white bars) or chondrogenic medium supplemented with 50 μg/ml of GY785 DRS (black bars) for 0, 14, and 28 days. Expression of COL2A1, ACAN, SOX9, COMP, COL1A1, and COL10A1 was investigated by real-time RT-PCR. Results are expressed as relative expression level compared to the basal level at day 0 in the absence of polysaccharide. *p < .05 compared to the chondrogenic condition at the same day. Abbreviations: CH, chondrogenic; CT, control.