Fig. 3.

Changes in phosphorylation status of different proteins upon DPPB treatment. (A), (C) EA.hy926 cells or (B), (D) HUVECs were treated with 10 μM of DPPB or solvent vehicle for the indicated time points or for 24 h in figure (B) and (D) and subjected to Western blot and subsequent densitometric analysis for the detection and quantification of (phospho-) eNOS levels, (phospho-) Akt levels or (phospho-) AMPK levels, respectively. One representative blot is shown. Band intensities are normalized to tubulin or actin and expressed as fold activation in comparison to the untreated control (***p < 0.001; **p < 0.01; *p < 0.05; n.s., not significant) (mean ± SEM, n = 3).