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Ldb1/2 mutant embryos lack hindlimb formation. Ldb1fl/+;Ldb2+/- control embryo (a) compared to Ldb1fl/fl;Ldb2-/-;Tcre mutant embryo (b) at E13.5. Skeletal structure of a corresponding pair of control Ldb1fl/+;Ldb2+/- (c) and Ldb1fl/fl;Ldb2-/-;Tcre mutant embryos at E14(d). Embryos were stained with alizarin red and alcian blue. FL, forelimb; HL, hindlimb.
