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. Author manuscript; available in PMC: 2013 Sep 7.
Published in final edited form as: J Proteome Res. 2012 Aug 15;11(9):4722–4732. doi: 10.1021/pr300536k

Fig. 7. The measurement of Ub, Nedd8 and ISG15 in metabolically labeled mouse brain.

Fig. 7

(A). Light recombinant proteins were mixed with monomeric (less than 12 KDa) or conjugated (>12 KDa) metabolically labeled mouse cerebral cortex (heavy Lys, +6.0201 Da). These mixed proteins were digested with trypsin and analyzed on LC-MS to quantitate Ub and Nedd8 levels.

(B). The quantified protein level listed.