Table 2.
Eleven genes in which Conrad had unique SNPs vs. Sloan & Williams82
| GlymaID/Affy IDa | PFAMb | GO functionc | PANTHERd | KOGe | BLASTP | E-value | BLAST hit species | # of SNPs | SNP locations | IRf_C | IR_S |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Glyma19g40800/- |
WD domain, G-beta repeat |
- |
WD repeat protein |
WD-repeat protein WDR6, WD repeat superfamily |
Transducin/WD40 domain-containing protein |
0E+00 |
Arabidopsis thaliana |
47 |
exon, intron, downstream |
|
|
| Glyma19g40840/- |
Pectinesterase/Plant invertase/pectin methylesterase inhibitor |
Pectinesterase activity; cell wall; cell wall modification |
- |
- |
Pectinesterase; Pectinesterase inhibitor |
0E+00 |
Medicago truncatula |
1 |
downstream |
|
|
| Glyma19g40940/- |
Glycosyl hydrolases family 28 |
Carbohydrate metabolism; polygalacturonase activity |
- |
- |
Glycoside hydrolase family 28 protein |
0E+00 |
Arabidopsis thaliana |
2 |
upstream, intron |
|
|
| Glyma19g41590/ Gma.14131.1.S1_atGmaAffx.26456.1.S1_at GmaAffx.76884.1.S1_at |
Haloacid dehalogenase-like hydrolase|Redoxin|NHL repeat |
Hydrolase activity |
2-deoxyglucose-6-phosphate phosphatase 2 |
Predicted haloacid-halidohydrolase and related hydrolases |
2-deoxyglucose-6-phosphate phosphatase, putative |
0E+00 |
Ricinus communis |
1 |
intron |
- |
- |
| Glyma19g41900/- |
F-box domain |
- |
- |
- |
Phloem-specific lectin PP2-like protein |
2E-82 |
Arabidopsis thaliana |
8 |
upstream, 5' UTR, exon, downstream |
|
|
| Glyma19g42120/ Gma.14232.1.S1_at |
- |
- |
- |
Uncharacterized conserved protein |
heparan-alpha-glucosaminide N-acetyltransferase |
0+00 |
Arabidopsis thaliana |
1 |
intron |
−2,-3 |
−2,-3,-5 |
| Glyma19g42200/ Gma.9498.1.S1_a_at |
Rapid ALkalinization Factor (RALF) |
Signal transducer activity |
- |
- |
Rapid ALkalinization Factor |
9e-47 |
Medicago truncatula |
11 |
upstream, intron |
2 |
- |
| Glyma19g42210/ GmaAffx.69813.1.A1_at |
RAD9 |
DNA repair |
DNA repair protein RAD9 |
Checkpoint 9-1-1 complex, RAD9 component |
Rad9 |
0E+00 |
Medicago truncatula |
12 |
upstream, exon, intron |
- |
- |
| Glyma19g42220/ GmaAffx.33386.1.A1_at |
Respiratory burst NADPH oxidase; EF hand; Ferric reductase like transmembrane component; FAD-binding domain; Ferric reductase NAD binding domain |
Calcium ion binding iron ion binding oxidoreductase activity; FAD binding |
NADPH oxidase |
Ferric reductase, NADH/NADPH oxidase and related proteins |
Respiratory burst oxidase 2 |
0E+00 |
Medicago truncatula |
29 |
upstream, exon, intron |
2 |
2 |
| Glyma19g42240/ Gma.13144.1.S1_at |
Core histone H2A/H2B/H3/H4;Histone-like transcription factor (CBF/NF-Y) and archaeal histone |
DNA binding |
Histone H2A |
Histone 2A |
Histone H2A 7 |
2e-73 |
Arabidopsis thaliana |
2 |
upstream, 5' UTR |
−2,5 |
3,5 |
| Glyma19g42390/- | Cyclin, N-terminal domain | - | Family not named | Cyclin | Cyclin-dependent protein kinase, putative | 6e-59 | Ricinus communis | 8 | upstream, intron, downstream |
a GlymaID/Affymetrix probe IDs that match with the predicted genes underlying QTL (http://soybase.org/AffyChip/), where “-” means there was no Affymetrix IDs available for the specific gene;
b PFAM description provided by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);
c Gene Ontology Descriptions obtained from the (http://soybase.org, updated on July 2011);
d PANTHER description provided by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);
e KOG Description assigned by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);
f IR: Infection response—significant differences in transcript abundance of inoculated samples compared to mock-inoculated samples in Conrad (C) or Sloan (S) (microarray data, TST-FDR, P < 0.05) at specified time points (dai); where “-” means there was no significant response observed at any time point after inoculation, a positive value indicates that the gene was up-regulated at the specified time point, and a negative value indicates the gene was down-regulated at the specified time point [10,21].