Table 5.
GlymaID |
PFAMa |
GO functionb |
PANTHERc |
KOGd |
BLASTP |
E-value |
BLAST hit species |
Contrasts |
Sampling points (hai) |
||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
12 | 24 | 48 | 72_Ih | 72_Ui | |||||||||
Glyma19g35340 |
Zinc-binding dehydrogenase |
Zinc ion binding |
Alcohol dehydrogenase related |
Alcohol dehydrogenase, class III |
Alcohol dehydrogenase, putative |
0E + 00 |
Ricinus communis |
CCe |
|
|
|
|
|
|
|
|
|
|
|
|
|
RCf |
|
|
|
1.5 |
|
|
|
|
|
|
|
|
|
ICg |
|
|
|
|
|
Glyma19g40800 |
WD domain, G-beta repeat |
- |
WD repeat protein |
WD-repeat protein WDR6, WD repeat superfamily |
Transducin/WD40 domain-containing protein |
0E + 00 |
Arabidopsis thaliana |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
|
1.8 |
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g40940 |
Glycosyl hydrolases family 28 |
Carbohydrate metabolism; polygalacturonase activity |
- |
- |
Glycoside hydrolase family 28 protein |
0E + 00 |
Arabidopsis thaliana |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
−1.9 |
|
|
|
|
|
|
|
|
|
|
IC |
|
|
−1.6 |
|
|
Glyma19g40950 |
WRKY DNA -binding domain |
Transcription factor activity; sequence-specific DNA binding |
- |
- |
Putative WRKY transcription factor 42 |
9E-91 |
Arabidopsis thaliana |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
|
|
|
|
|
|
|
|
|
|
|
IC |
|
|
−1.5 |
|
|
Glyma19g40970 |
AUX/IAA family |
Transcription factor activity |
- |
- |
Auxin-responsive protein IAA20, putative |
1E-48 |
Ricinus communis |
CC |
−1.5 |
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
|
|
−1.9 |
|
|
|
|
|
|
|
|
IC |
|
−2.2 |
|
|
−1.8 |
Glyma19g41580 |
- |
- |
- |
- |
Transcription factor bHLH149 |
1E-30 |
Arabidopsis thaliana |
CC |
|
|
−1.6 |
−2.8 |
|
|
|
|
|
|
|
|
|
RC |
|
|
1.5 |
2.3 |
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g41780 |
GATA zinc finger |
Transcription factor activity; regulation of transcription, DNA-dependent; zinc ion binding; sequence-specific DNA binding |
Transcription factor gata (gata binding factor) |
- |
GATA transcription factor 16 |
1E-23 |
Arabidopsis thaliana |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
−1.5 |
|
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g41800 |
Kinesin motor domain |
ATPase activity microtubule binding microtubule motor activity |
Kinesin heavy chain |
Kinesin (KAR3 subfamily) |
Kinesin heavy chain, putative |
0E + 00 |
Ricinus communis |
CC |
−5.1 |
−5.6 |
−6.7 |
−2.9 |
−3.3 |
|
|
|
|
|
|
|
|
RC |
|
|
|
2.0 |
|
|
|
|
|
|
|
|
|
IC |
−5.4 |
−6.2 |
−7.1 |
−6.2 |
−2.7 |
Glyma19g41870 |
Protein phosphatase 2C |
Protein serine/threonine phosphatase activity |
Protein phosphatase 2c |
Serine/threonine protein phosphatase |
Protein phosphatase 2c, putative |
0E + 00 |
Ricinus communis |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
1.6 |
|
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g41900 |
F-box domain |
- |
- |
- |
Phloem-specific lectin PP2-like protein |
2E-82 |
Arabidopsis thaliana |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
1.9 |
|
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g41930 |
Leucine Rich Repeat |
Protein binding |
F-box/leucine rich repeat protein |
Leucine rich repeat proteins, some proteins contain F-box |
Ubiquitin-protein ligase, putative |
0E + 00 |
Ricinus communis |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
|
2.1 |
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g42050 |
Calcineurin-like phosphoesterase |
Protein serine/threonine phosphatase activity |
Serine/threonine protein phosphatase |
Serine/threonine specific protein phosphatase PP1, catalytic subunit |
Serine/threonine-protein phosphatase PP1 isozyme 8 |
0E + 00 |
Arabidopsis thaliana |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
1.8 |
|
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g42120 |
- |
- |
- |
Uncharacterized conserved protein |
heparan-alpha-glucosaminide N-acetyltransferase |
0 + 00 |
Arabidopsis thaliana |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
|
2.0 |
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g42200 |
Rapid ALkalinization Factor (RALF) |
Signal transducer activity |
- |
- |
Rapid ALkalinization Factor |
9e-47 |
Medicago truncatula |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
1.6 |
|
|
|
|
|
|
|
|
|
|
IC |
|
|
|
|
|
Glyma19g42340 |
Protein tyrosine kinase |
Protein-tyrosine kinase activity; protein amino acid phosphorylation; ATP binding |
Mapkk-related serine/threonine protein kinases |
MEKK and related serine/threonine protein kinases |
Mitogen activated protein kinase kinase kinase 3, mapkkk3, mekk3, putative |
0E + 00 |
Ricinus communis |
CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
RC |
|
|
|
2.8 |
|
IC | 2.1 | 2.0 |
R group: lines with the Conrad haplotype (Conrad, the RILs 2022, and 1960); S group: lines with the Sloan haplotype (Sloan, the RILs 1974, and 2022). Empty cell indicates that there was no significant difference, and a negative value indicates the fold of greater expression ratio in the group with the Sloan haplotype.
a PFAM description provided by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);
b Gene Ontology Descriptions obtained from the (http://soybase.org, updated on July 2011);
c PANTHER description provided by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);
d KOG Description assigned by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);
e CC: Constitutive contrast—significant fold difference in transcript abundance between the group with Conrad haplotype and the group with Sloan haplotype in mock-inoculated samples at specified time points (hai);
f RC: Response contrast—significant fold differences in infection response ratios (inoculated / mock-inoculated) in the group with Conrad haplotype compared to the group with Sloan haplotype at specified time points (hai);
g IC: Infection contrast—significant fold difference in transcript abundance between the group with Conrad haplotype and the group with Sloan haplotype in infected samples at specified time points (hai);
h Samples were collected from the inoculation site at 72 HAI;
i Samples were collected from the front of lesion margin at 72 HAI.