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. 2012 Aug 28;13:428. doi: 10.1186/1471-2164-13-428

Table 5.

Genes with significant expression differences in the R group vs. S group in qRT-PCR.P< 0.05, Fold difference > 1.5

GlymaID
PFAMa
GO functionb
PANTHERc
KOGd
BLASTP
E-value
BLAST hit species
Contrasts
Sampling points (hai)
                  12 24 48 72_Ih 72_Ui
Glyma19g35340
Zinc-binding dehydrogenase
Zinc ion binding
Alcohol dehydrogenase related
Alcohol dehydrogenase, class III
Alcohol dehydrogenase, putative
0E + 00
Ricinus communis
CCe
 
 
 
 
 
 
 
 
 
 
 
 
 
RCf
 
 
 
1.5
 
 
 
 
 
 
 
 
 
ICg
 
 
 
 
 
Glyma19g40800
WD domain, G-beta repeat
-
WD repeat protein
WD-repeat protein WDR6, WD repeat superfamily
Transducin/WD40 domain-containing protein
0E + 00
Arabidopsis thaliana
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
 
1.8
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g40940
Glycosyl hydrolases family 28
Carbohydrate metabolism; polygalacturonase activity
-
-
Glycoside hydrolase family 28 protein
0E + 00
Arabidopsis thaliana
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
−1.9
 
 
 
 
 
 
 
 
 
 
IC
 
 
−1.6
 
 
Glyma19g40950
WRKY DNA -binding domain
Transcription factor activity; sequence-specific DNA binding
-
-
Putative WRKY transcription factor 42
9E-91
Arabidopsis thaliana
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
 
 
 
 
 
 
 
 
 
 
 
IC
 
 
−1.5
 
 
Glyma19g40970
AUX/IAA family
Transcription factor activity
-
-
Auxin-responsive protein IAA20, putative
1E-48
Ricinus communis
CC
−1.5
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
 
 
−1.9
 
 
 
 
 
 
 
 
IC
 
−2.2
 
 
−1.8
Glyma19g41580
-
-
-
-
Transcription factor bHLH149
1E-30
Arabidopsis thaliana
CC
 
 
−1.6
−2.8
 
 
 
 
 
 
 
 
 
RC
 
 
1.5
2.3
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g41780
GATA zinc finger
Transcription factor activity; regulation of transcription, DNA-dependent; zinc ion binding; sequence-specific DNA binding
Transcription factor gata (gata binding factor)
-
GATA transcription factor 16
1E-23
Arabidopsis thaliana
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
−1.5
 
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g41800
Kinesin motor domain
ATPase activity microtubule binding microtubule motor activity
Kinesin heavy chain
Kinesin (KAR3 subfamily)
Kinesin heavy chain, putative
0E + 00
Ricinus communis
CC
−5.1
−5.6
−6.7
−2.9
−3.3
 
 
 
 
 
 
 
 
RC
 
 
 
2.0
 
 
 
 
 
 
 
 
 
IC
−5.4
−6.2
−7.1
−6.2
−2.7
Glyma19g41870
Protein phosphatase 2C
Protein serine/threonine phosphatase activity
Protein phosphatase 2c
Serine/threonine protein phosphatase
Protein phosphatase 2c, putative
0E + 00
Ricinus communis
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
1.6
 
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g41900
F-box domain
-
-
-
Phloem-specific lectin PP2-like protein
2E-82
Arabidopsis thaliana
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
1.9
 
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g41930
Leucine Rich Repeat
Protein binding
F-box/leucine rich repeat protein
Leucine rich repeat proteins, some proteins contain F-box
Ubiquitin-protein ligase, putative
0E + 00
Ricinus communis
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
 
2.1
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g42050
Calcineurin-like phosphoesterase
Protein serine/threonine phosphatase activity
Serine/threonine protein phosphatase
Serine/threonine specific protein phosphatase PP1, catalytic subunit
Serine/threonine-protein phosphatase PP1 isozyme 8
0E + 00
Arabidopsis thaliana
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
1.8
 
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g42120
-
-
-
Uncharacterized conserved protein
heparan-alpha-glucosaminide N-acetyltransferase
0 + 00
Arabidopsis thaliana
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
 
2.0
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g42200
Rapid ALkalinization Factor (RALF)
Signal transducer activity
-
-
Rapid ALkalinization Factor
9e-47
Medicago truncatula
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
1.6
 
 
 
 
 
 
 
 
 
 
IC
 
 
 
 
 
Glyma19g42340
Protein tyrosine kinase
Protein-tyrosine kinase activity; protein amino acid phosphorylation; ATP binding
Mapkk-related serine/threonine protein kinases
MEKK and related serine/threonine protein kinases
Mitogen activated protein kinase kinase kinase 3, mapkkk3, mekk3, putative
0E + 00
Ricinus communis
CC
 
 
 
 
 
 
 
 
 
 
 
 
 
RC
 
 
 
2.8
 
                IC       2.1 2.0

R group: lines with the Conrad haplotype (Conrad, the RILs 2022, and 1960); S group: lines with the Sloan haplotype (Sloan, the RILs 1974, and 2022). Empty cell indicates that there was no significant difference, and a negative value indicates the fold of greater expression ratio in the group with the Sloan haplotype.

a PFAM description provided by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);

b Gene Ontology Descriptions obtained from the (http://soybase.org, updated on July 2011);

c PANTHER description provided by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);

d KOG Description assigned by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php, updated on July 2011);

e CC: Constitutive contrast—significant fold difference in transcript abundance between the group with Conrad haplotype and the group with Sloan haplotype in mock-inoculated samples at specified time points (hai);

f RC: Response contrast—significant fold differences in infection response ratios (inoculated / mock-inoculated) in the group with Conrad haplotype compared to the group with Sloan haplotype at specified time points (hai);

g IC: Infection contrast—significant fold difference in transcript abundance between the group with Conrad haplotype and the group with Sloan haplotype in infected samples at specified time points (hai);

h Samples were collected from the inoculation site at 72 HAI;

i Samples were collected from the front of lesion margin at 72 HAI.