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. 2012 May 4;12:18. doi: 10.1186/1472-6750-12-18

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Copyright ©2012 Cardoso et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Photomicrographs representative of the morphological appearance of chondrogenic, osteogenic and adipogenic differentiation of bovine-derived UC-WJ cells at P40. For each differentiation protocol, undifferentiated cells were kept as controls ( D, E, F). A) Presence of calcium mineralization after 20 d of induction Alizarin Red staining; B) Presence of acidic proteoglycans were observed after 1 week of chondrogenic differentiation by Safranin O staining; C); adipogenic differentiation after 15 d showing lipid droplets stained with Oil Red (scale bar: 20 μm) ( D); after neurogenic induction of 2 week a neuronal-like morphology was visible under immunofluorescence microscopy positive N200 cell marker ( E) (scale bar: 20 μm).