Figure 5.

Flow cytometric analyses of surface marker expression and neuronal induction in bovine-derived UC-WJ cells.A).The data of negative isotype control and respective surface markers for mesenchymal stromal cells are shown by histogram. B) The data shown are the representative cell phenotype after neuronal induction. The data was processed by Attune™ acoustic focusing cytometer and excluded auto-fluorescence as a global compensation tool (≤ 10³). Y-axis represents log scale; C) After neurogenic induction of 2 week a neuronal-like morphology was visible under immunofluorescence microscopy positive N200, CXR4, GFAP and SNAP-25 cell markers (scale bar: 20 μm).