Figure 2. Excision of exons 7 and 8 of Atbf1 by the Cre recombinase.

a. Schematic representation of Cre-mediated deletion of exons 7 and 8. LoxP and Frt sequences are marked by triangle and diamond-shaped boxes respectively. Primers F1 and R2 are indicated by arrow heads. The Atbf1^Δ7&8 deletion allele was induced by Cre from adenoviruses or EIIa-cre mice. b. Five MEF clones incubated with adenoviruses expressing GFP or Cre were genotyped by a PCR using primers F1 and R2, identifying three Atbf1 alleles: Atbf1^flox (1248 bp), Atbf1^wt (1071 bp) and Atbf1^Δ7&8 (289 bp). c. Detection of the wildtype (532 bp) and truncated (197 bp) Atbf1 mRNA by RT-PCR in MEFs with different status of Atbf1 deletion. d. Detection of Atbf1 protein in MEFs with different Atbf1 deletion status by immunoblotting. Molecular masses of protein standards are shown to the left of the gel.