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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Kidney Int. 2012 Jun 13;82(8):878–891. doi: 10.1038/ki.2012.224

Figure 1. A1AR activation or overexpression increases sphingosine kinase (SK)-1 expression and activity in mouse kidney.

Figure 1

Figure 1

Figure 1

A. Representative bands for SK1 and SK2 mRNA (RT-PCR, 6 hr) and protein (immunoblotting, 16 hr) expression in mouse kidney treated with vehicle (0.4% DMSO in saline) or CCPA (0.1 mg/kg, i.p.) (N=4 for each group). B. Mouse kidney SK activity 6 hr after vehicle (0.4% DMSO) or CCPA (0.1 mg/kg, i.p.) treatment (N=4 for each group). Data are presented as means ± SEM. *P<0.05 vs. vehicle-treated mice. C. Representative bands for SK1 and SK2 mRNA (RT-PCR) and SK1 protein (immunoblotting) expression in mouse kidney injected with EGFP or A1AR-EGFP lentivirus 48 hr prior (N=4 for each group).