Table 1.

Primers used to amplify mRNAs based on published GenBank sequences for mice.

Primers	Accession Number	Sequence (Sense/Antisense)	Product Size (bp)	Cycle Number	Annealing Temp (°C)
Mouse S1P1R	NM_007901	5′-CGGTGTAGACCCAGAGTCCT-3′ 5′-AGCAGCAGATGAGAATGAAC-3′	393	20	64
Mouse S1P2R	NM_010333	5′-AAAACCAACCACTGGCTGTC-3′ 5′-GAGTGGAACTTGCTGTT-3′	317	25	60
Mouse S1P3R	NM_010101	5′-AAGCCTAGCGGGAGAGAAAC-3′ 5′-GGCAATCAAAACCATCAGGT-3′	452	22	64
Mouse S1P4R	NM_010102	5′-GCAGAAGTCTCCACGTCCTC-3′ 5′-GCTGAGTGACCGAGAAGTCC-3′	403	23	62
Mouse S1P5R	NM_053190	5′-ACACCAAATGCCCAGCTTAC-3′ 5′-ACCAAGAGCACAGCCAAGTTC-3′	335	32	62
Mouse ICAM-1	X52264	5′-TGTTTCCTGCCTCTGAAGC-3′ 5′-CTTCGTTTGTGATCCTCCG-3′	409	21	60
Mouse TNF-α	X02611	5′-TACTGAACTTCGGGGTGATTGGTCC-3′ 5′-CAGCCTTGTCCCTTGAAGAGAACC-3′	290	24	65
Mouse HSP27	NM_024441	5′-CCTAAGGTCTGGCATGGTA-3′ 5′-AGGAAGCTCGTTGTTGAAGC-3′	373	25	66
Mouse SK1	NM_011451 (variant 1) NM_025367 (variant 2)	5′-GATGCATGAGGTGGTGAATG-3′ 5′-GCCCACTGTGAAACGAATC-3′	337	22	64
Human SK1	NM_021972 (variant 1) NM_182965 (variant 2) NM_001142601 (variant 3) NM_001142602 (variant 4)	5′-ATCTCCTTCACGCTGATGC-3′ 5′-GTGCAGAGACAGCAGGTTCA-3′	330	26	66
Human SK2	NM_020126 (variant 1) NM_001204158 (variant 2) NM_001204159 (variant 3) NM_001204160 (variant 4)	5′-GGAGGAAGCTGTGAAGATGC-3′ 5′-GCAGGTCAGACACAGAACGA-3′	482	22	66
Mouse and human GAPDH	M32599	5′-ACCACAGTCCATGCCATCAC-3′ 5′-CACCACCCTGTTGCTGTAGCC-3′	450	15	65
Cre transgene		5′-TGGGCGGCATGGTGCAAGTT-3′ 5′-CGGTGCTAACCAGCGTTTTC-3′	466	30	61
S1P1RloxP		5′-GAGCGGAGGAAGTTAAAAGTG-3′ 5′-CCTCCTAAGAGATTGCAGCAA-3′	WT: 200 LOX: 250	45	56

bp, base pairs; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HSP27, heat shock protein 27; ICAM-1, intercellular adhesion molecule-1; S1PR, sphingosine 1-phosphate receptor; SK, sphingosine kinase; TNF-α, tumor necrosis factor alpha.