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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Cancer Res. 2012 Jun 6;72(16):3948–3957. doi: 10.1158/0008-5472.CAN-11-4134

Figure 2. Functional evaluation of proposed p53 response elements (REs) in TLR promoters.

Figure 2

A. SaOS2 cells were transfected with luciferase reporter constructs containing p53 RE sequences from different TLRs in the presence (solid bars) or absence (open bars) of the vector expressing wild type p53. At 48 h post-transfection, induction of the luciferase reporter was assessed. Relative luciferase activity was compared to the pGL4 plasmid lacking the p53 RE (empty vector) or constructs that contained REs of established p53 targets. Shown is an average of three independent experiments each performed in triplicate. B. MCF7 cells were treated with 0.3 µg/ml of doxorubicin (dxr) or 10 µM of Nutlin-3 for 24 h. Occupancy of p53 at promoters of TLRs 24 h later was assessed by ChIP assay and quantified by qPCR.