Figure 6. Mutations in p53 differentially modify p53 effects on expression of the TLR gene family.
A. p53 null SaOS2 cells were transfected with a panel of cancer-related p53 mutants. At 24 h post-transfection, cells were harvested and p53 nuclear levels were evaluated by Western blot. B. Presented is a heat map of fold-change in mRNA expression for TLRs, p21 and B2M genes following transfection with WT or mutant p53 as compared to the mock transfected control (set as one). Gene expression was assessed by real time PCR and normalized to GAPDH expression.