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. Author manuscript; available in PMC: 2012 Oct 7.
Published in final edited form as: J Proteome Res. 2011 Sep 23;10(10):4624–4633. doi: 10.1021/pr200434y

Figure 1.

Figure 1

Isolation of exosomes and cell membrane for glycomic comparison. (A) Schematic of microvesicles and membrane isolation. (B) Western blots of the microvesicle preparations (MV) from HCT-15 (HCT), HT29 (HT), SkMel-5 (SkM), Jurkat-TAT-CCR5 (Jurk), H9, Sup-T1 (SupT), and breast milk (BrM). Markers CD63 and CD81 are present in all samples. (C) Equal amounts (3 ug protein) of SkMel-5 MV and cell membrane (MB) were probed for CD63 and CD81. (D) Transmission electron microscopy images of SkMel-5 MV and MB. Bar is 200 nm.