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. Author manuscript; available in PMC: 2013 Mar 1.

Published in final edited form as: Protein Expr Purif. 2011 Dec 8;82(1):106–115. doi: 10.1016/j.pep.2011.11.016

Table II.

Direct comparison of Avi-GCPII and rhGCPII enzymatic activity.

	Avi-GCPII			rhGCPII

Substrate	K_m [µmol/L]	k_cat [per second]	k_cat/K_m [10⁵/s.(mol/L)]	K_m [µmol/L]	k_cat [per second]	k_cat/K_m [10⁵/s.(mol/L)]
Ac-Asp-Glu	0.53 ± 0.08^*	0.46 ± 0.01^*	8.66 ± 1.4	1.06 ± 0.18^*	0.41 ± 0.02^*	3.88 ± 1.06
Ac-Asp-Met	20.8 ± 3.3^§	0.29 ± 0.01^§	0.14 ± 0.02	25.2 ± 4.0^§	0.34 ± 0.02^§	0.13 ± 0.02

Inhibitor	K_i [pmol/L]			K_i [pmol/L]

2-PMPA	370 ± 80^§			540 ± 120^§

^*

measurements were performed by radioactive assay

^§

measurements were performed by HPLC assay