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. Author manuscript; available in PMC: 2012 Sep 17.
Published in final edited form as: J Acquir Immune Defic Syndr. 2010 Sep;55(1):87–94. doi: 10.1097/QAI.0b013e3181e53c64

Figure 2.

Figure 2

Figure 2

(A) Binding assay of fluorescently labelled zymosan to the wild-type (wt) and the 223S mutated (mut) form of dectin-1 cloned and transfected with an hemagglutinin tag in NIH3T3 cells, including competitive blocking by laminarin, on the dectin-1 isoforms A and B. Values are relative fluorescent units (RFU). (B) Evaluation of membrane expression of the cloned dectin-1 constructs, wild-type (wt) and 223S mutant (mut) of both isoforms A and B, transfected with an hemagglutinin tag in NIH3T3 cells and measured by using HA-antibodies and flow cytometry.