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. Author manuscript; available in PMC: 2012 Sep 17.
Published in final edited form as: Mol Cell. 2011 Jan 7;41(1):46–55. doi: 10.1016/j.molcel.2010.12.008

Figure 5. APLF binds to macroH2A1 and shuttles its macro domain to the sites of DNA damage.

Figure 5

Binding of APLF to histones is altered in response to DNA damage. This effect can be blocked by inhibiting active poly(ADP-ribose) synthesis. Complexes were purified by immunoprecipitation of transiently expressed FLAG-tagged APLF in HEK293T cells and analyzed by immunobloting. B, Recruitment of fluorescently tagged macroH2A1 macro domain, ALC1 and XRCC1 to the sites of laser-induced DNA damage in APLF WT and APLF− cells. Recruitment of macroH2A1 macrodomain to sites of DNA damage is dependent on presence of of APLF. C, As for B, but in the presence of PARP inhibitor.