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ABF1 binding to HMLα I silencer requires an ABF1-binding site. Immunoprecipitation with ABF1 antibody was performed in RAD+ and RAD+/Abf1bs cells. The occupancy of ABF1 was measured by qPCR with the primers spanning the HMLα I silencer region (qPCR1, qPCR4, and qPCR5). Data are represented as average of three experiments ±S.D.
