Figure 1.

(a) Schematic of the micropipette adhesion frequency assay. Dimeric WT or mutant PSGL-1 was expressed on transfected CHO cells expressing FucTVII and C2nT. Soluble P-selectin or L-selectin-Ig chimera was coupled to an RBC with uniform orientation by capturing antibodies (S12 or AP113) precoated by CrCl₃ coupling. (b) PSGL-1 mutants. PSGL-1 was mutated by replacing two of the three (double mutants) or all three (triple mutant) sulfated tyrosines at residues 46, 48, and 51 by phenylalanines. Only the first 17 amino acids of mature PSGL-1 are shown.