Figure 3.

Ca⁺² influx into LUVs after the addition of freshly dissolved Aβ_1-40. The maximum values of Ca⁺² ion influx detected by encapsulated Fura-2 after 30 min of incubation with freshly dissolved Aβ_1-40 from 0.2 mg/ml TLBE LUVs (dark gray bars) or 0.2 mg/ml POPC/POPS 7:3 LUVs (light gray bars) are shown. In contrast to the 6-carboxyfluorescein dye release assay, Ca⁺² influx occurs shortly after the addition of Aβ_1-40 and occurs weakly in the absence of gangliosides. The antimicrobial peptide MSI-78 was used as a reference for total disruption of the membrane (black bar).