Figure 5.

Zn²⁺ ions cannot block the fiber-dependent step of membrane disruption. The figure indicates the influx of Ca⁺² (red line) and Zn²⁺ (blue line) ions induced by adding freshly dissolved Aβ_1-40 to 0.2 mg/ml TLBE LUVs incubated with preformed fibers. Freshly dissolved Aβ_1-40 was added to each sample at time zero, and Ca⁺² or Zn⁺² was added at 600 s as indicated by the dashed line. No Ca⁺² influx was detected after the addition of preformed Aβ_1-40 fibers (black line). The influx of both Ca⁺² and Zn²⁺ ions (red and blue lines, respectively) were detected by seeding Aβ_1-40 fiber formation with preformed fiber. This finding suggests that the fiber-dependent step of membrane disruption is not correlated with pore formation. LUVs were made from TLBE lipids.